? Flow Cytometry is the technology that measures properties of single particles and/or cells.
? Measures fluorescence, fire up scatter, and other properties of particles and/or cells.
? Can provide correlated data that links different population profiles all based on single particles and/or cells abbreviation (hydro-dynamic focusing).
What can Flow Cytometry do?
? Enumerate particles and/or cells in suspension.
? Determine biological from Non-biological samples.
? order life from dead cells.
? Evaluate 105 to 107 particles and/or cells in about one minute.
? Measure particles and/or cells scatter as well as innate fluorescence or 2° fluorescence.
? potpourri single particles and/or cells for sub-sequent analysis.
? Do advance multi-parameter analysis on complex data.
What are the principles?
? Light scattered by optical maser or arc lamp.
? Specific fluorescence detection.
? Hydro-dynamically focused stream of particles and/or cells.
? Electrostatic particles and/or cells separation for sorting.
? Multivariate data analysis capability.
Definitions
? Flow Cytometry (cyto = cell) (metry = measurement).
o Cyto is derived from the Greek kitos meaning hollow as a cell or container.
From the same root go in the combining form cyto and the suffix cyte which similarly denote a cell.
o Metry; Greek metria, from metron measure. Indicating the process of scientific measurement.
Technical Components
? catching Systems:
o Photomultiplier Tubes (PMTs).
o Diodes light scatter detectors.
? Illumination Systems:
o Lasers.
o Arc lamps.
Concepts
? Light Scatter:
o onwards Scatter roughly size for some particles and/or cells although it is a complex parameter 90° scatter shape, granularity, Polarized Scatter (birefringence) Structure.
o Materials scatter light at wavelengths at which they do...If you want to buy off a full essay, order it on our website: Ordercustompaper.com
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